Home / Biyoloji (page 3)


In-house NGS Plasmid Quality Control Now at Addgene

Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017 for all incoming plasmids was a big change for Addgene – we had used Sanger sequencing for quality control for over ten years. Now, thanks to a generous donation by the Hirsh …

Read More »

Weekly reads: stem cell videos, cystic fibrosis, papers

As part of our weekly reads I’m including one of my latest videos in our Stem Cell Channel on YouTube below. This particular video fact-checks stem cell face creams, but I’ve got quite a few more including on stem cell supplements, different kinds of stem cells and more. You might …

Read More »

Preventing ice contamination in the cryo-ET workflow

Cryogenic electron tomography (cryo-ET) is an imaging technique that allows reconstruction of high-resolution models of protein complexes in their near-native state. In cryo-ET, a sample (e.g. eukaryotic cells grown on an EM grid) is first vitrified, then thinned to the appropriate thickness (100-300 nm) using a Focussed Ion Beam (FIB) …

Read More »

Participate in the Pets of Addgene Scavenger Hunt

No pranks, just fun and games this April Fool’s Day on the Addgene blog.  We’ve been mostly working remotely for the last year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more time with our pets. These new “co-workers” …

Read More »

Perspectives on BioLife: one name, distinct companies

In the stem cell world some corporate names end up being very similar with the latest example being several companies with the name “BioLife.” Get a BioLife…name I guess BioLife is a catchy term. You’ve got life and biology in there in one name. Let’s deconstruct what’s going on out …

Read More »

Tailoring staining protocols to electron microscopy workflows

In EM, contrast is generated when electrons interact with the sample. However, most biological material consists of light elements such as carbon, oxygen, and nitrogen, which interact with the electron beam only sparsely. To enhance the contrast of specimens and enable room-temperature EM imaging, contrasting agents are introduced. These react …

Read More »